Therefore, the primary aim of this project was to find out more about the sex determination of this species. Zebrafish androgen receptor: Isolation, molecular, and biochemical characterization1. Oncotarget9— Originally, aCGH was developed for the analysis of chromosomes aberrations in cancer cells .
This experiment was carried out with the aim to determine if sex-biased ratios in lines can be maintained or increased by selecting for breeding pairs that produced brood with highly skewed sex ratios through several generations. Examples of other environmental cues that also have an influence on sex include pH in guppy  and social interactions in some reef fishes .
Grunwald D, Eisen J Headwaters of the zebrafish emergence of a new 1. CNV regions selected for further validation due to their apparent association to sex based on preliminary aCGH. It was considered preferable to split linkage groups where there were large gaps between markers, rather than join potentially independent linkage groups.
It was also observed that exposure to increased opinion, these two situations are principally the same, as there are temperature 35—37uC either during early development 5—48 hpf several genes distributed throughout the genome with major  or between 17—27 dpf  resulted in male-biased sex ratio.
J Exp Zool Part A. Even if there are sex chromosomes in zebrafish, they will be likely showing limited differences at sequence level and therefore undergo recombination with each other along the majority of their length. This indicates that the wide-ranging sex ratios normally observed are most likely due to the parental genotypes.
As a consequence, it should show different copy numbers between the two sexes; hence, it is possible to search for such sex chromosomes using aCGH. Inferring the type of sex chromosomal system by progeny sex ratio as a result of genome manipulation or artificial sex reversal.
The master sex-determination locus in threespine sticklebacks is on a nascent Y chromosome. Taken together, they seem to argue against the presence of a strong sex chromosomal system with primary effect on SD in zebrafish. Moving up the hierarchy: A hypothesis on the evolution of a genetic sex determination pathway.
Juvenile hermaphroditism in the zebrafish, Brachydanio rerio. The parents and four of their offspring from each cross were analyzed. Most data indicate that SD in zebrafish is not based primarily on the action of sex chromosomes.
Nucleic Acids Res. Genetica — Since in mammals, including humans, sex is determined by CSD, the vast majority of our knowledge on the molecular regulation of vertebrate sex is based on data collected from such systems.